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Using a forced-air incubator The thermo-responsive characteristics (i.e., phase separation property) of the L31, L35 and L61 copolymer solutions at various concentrations (580 wt.%) were studied by heating or cooling the solution using a temperature controlled water bath (heater Julabo SW22, Germany and chiller Xtemp XT5618B, China). Find your water bath Use a water bath to heat tubes for either Standard (37C incubation) or Enhanced (60C incubation) Protocol. 60C water bath is needed Reducing sugars and reducing agents Thiols Copper-chelating agents Ascorbic acid and uric acid Tyrosine, cysteine, and tryptophan 50 mM imidazole Thermo Scientific Pierce Modified Lowry Protein Assay Kit Standard protocol: 11,500 g/mL Microplate protocol: 101,500 g/mL (40 L) Using a forced-air incubator Furnace Accessories. After sonication, the suspension becomes highly dispersed. The first is the T/C (thermal conductivity) sensor. 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